Use of organic fertilizer are getting more stressed recently, in view of recycling of resource and maintenance of environment. Grafting and facilities culture are generally adopted to increase resistance to disease and quality of bitter gourd. Nevertheless, facilities soil are frequently deteriorated and unable for sustainable use, due to salt accumulated problems, caused by high temperature and lack of leaching by precipitation. In this study, facilities culture with bitter gourd seedlings (Momordica charantia L.) was conducted in a field ( clay slate non-calcareous alluvial soil,TSp2), located in Miaoli Wanli. Three batches of compost, composted at different periods but with the same material, were applied in spring and summer crops, while three kinds of manure( chicken, cattle, and cattle-hog mixed manure)were used in autumn and winter crops. Besides, treatments of chemical fertilizer non-fertilizer, as check plots, were involved to investigate the effects of organic fertilizer prepared from different sources and compositions on soil fertility as well as growth, yield and quality of bitter gourd.