英文摘要 |
The village Putai was one of the four main “blackfoot disease”(BFD) endemic area located on the southwest coast of Taiwan. There have been many studies on accumulation and release of arsenic in sediments in these areas, but geomicrobiologic study is scarce. Sulfate-reducing bacteria contribute a diverse group of prokaryotes that contribute a variety of essential functions in many anaerobic environments. Recently, many studies suggest that sulfate reducers such as Desulfovibrio spp. and Desulfomicrobium spp. might be responsible for arsenate reduction in sediments, and certain sulfate-reducing bacteria might be able to reduce humic substances.
This study aims at revealing the geochemical characteristics of sediments and porewater from a core drilling at Putai. Using molecular biological method, DNA was extracted from sediments. PCR primer sets for the 16S rRNA gene of specific sulfate-reducing bacteria were designed to identify principal sulfate-reducing bacteria related to the reduction of arsenic and humic substances, in sediments with higher concentration of arsenic and humic substances. |